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Greiner Bio
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Revvity
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Greiner Bio
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fluidigm
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Image Search Results
Journal: ASN NEURO
Article Title: Advancements in a FRET Biosensor for Live-Cell Fluorescence-Lifetime High-Throughput Screening of Alpha-Synuclein
doi: 10.1177/17590914231184086
Figure Lengend Snippet: Thiot fibrillization extent. All 25 compounds with FLT-FRET dose response plus the control EGCG were tested in a seeded ThioT aggregation assay. (a) A representative ThioT trace showing vehicle (DMSO, green), hit compounds EGCG, proanthocyanidins, casanthranol, (inhibitors of aggregation), and Paroxetine HCl (compound with no effect, purple). Inset is a magnification of the dashed box showing rapid inhibition by EGCG and proanthocyanidins at early time points. (b and c) In total, 18 compounds resulted in significant attenuation of aSyn seeded fibrillization (1 at p < .05; 1 at p < .005; and 16 at p < .001). Full kinetic traces for each hit are presented in Supplemental Figure S10 . Data represents mean fibrillization extent and SEM from N = 2 independent experiments with three replicate wells per experiment.
Article Snippet: 50 μl of the
Techniques: Control, Inhibition